Abstract Detail

Mycology

Wallace, Emma [1], Jimenez-Gasco, Maria del Mar [2], Geiser, David [2].

Global diversity and population trends of Fusarium oxysporum isolated from agricultural and non-agricultural niches.

Fusarium oxysporum  (Fo) is an ubiquitous group comprising plant pathogens, saprotrophs, endophytes, and even agents of opportunistic human infection. Lack of a clear picture of the complex evolutionary patterns underlying ecological functions in  Fo  is a major barrier to our understanding of how pathogenicity emerges and developing robust diagnostics. Although plant pathogenic  Fo  have been well-characterized, studies examining isolates from soil and asymptomatic plants are underrepresented, which potentially distorts our view of  Fo  evolution. Putatively nonpathogenic isolates display high diversity, endemism, and suggest adaptation to a particular host. However, assumptions about nonpathogenic  Fohave often been made from isolated observations. To provide an accessible, comprehensive summary of  Fo  diversity, we systematically analyzed data from nine published studies on nonpathogenic  Fo. Specifically, we made comparisons between Fo datasets from uncultivated ecosystems, tomato agroecosystems, and banana agroecosystems. Datasets include metadata of  Fo  across five continents connected to translation elongation factor 1-    (tef1) marker sequences, which serve as a barcode for the genus  Fusarium. We evaluated phylogenetic relationships between isolates by geography (country), ecosystem type (agroecosystem, uncultivated ecosystem), and substrate (soil, tomato root, tomato stem, banana root, banana stem). Frequency and distribution of  tef1  sequence types across these factors are also examined and sequences from other informative regions are compared when possible. Examining these datasets from a global perspective provides a picture of  Fo  diversity in different ecological niches. The resulting sequence database is useful for broader examinations of host adaptation within  Fo  and will facilitate connections between diverse studies on this globally important group.

1 - Penn State University, Plant Pathology and Environmental Microbiology, 211 Buckhout Laboratory, University Park, PA, 16802, USA
2 - Penn State University, Plant Pathology and Environmental Microbiology, 211 Buckhout Laboratory, University Park, PA, 16803, USA

Keywords:
Fusarium oxysporum
Endophytes
Mycology
aysmptomatic.

Presentation Type: Poster
Session: MYP2, Mycology Posters II
Location: Virtual/Virtual
Date: Tuesday, July 20th, 2021
Time: 5:00 PM(EDT)
Number: MYP2014
Abstract ID:900
Candidate for Awards:MSA Best Poster Presentation Award by a Graduate Student