| Abstract Detail
Comparative Genomics/Transcriptomics Rahmatpour, Nasim [1], Kuo, Li-Yaung [2], Kang, Jessica [3], Herman, Elaina [3], Zhou, Junhui [3], Wolniak, Stephen [3], Zipper, Richard [4], Delwiche, Charles [5], Mount, Stephan M [6], Li, Fay-Wei [7]. A chromosome-scale genome assembly of the fern Marsilea vestita. To date, only two fern genomes have been published,neither of which was assembled at the chromosomal level. With such a limited genomic resource, it has been difficult to gain a holistic view of the fern genome space. Here, we report the genome assembly and annotation of Marsilea vestita (Marsileaceae), which has been a model for male gametophyte development, including posttranscriptional regulation and intron retention. Using a combination of nanopore long reads, Illumina short reads, and high-throughput chromosome conformation capture (Hi-C), we assembled the genome into 20 chromosomes. We annotated the genome based on protein evidence and 18 RNA-seq libraries from leaf tissues, as well as different time intervals of spermatogenesis and treatments. Our data provides a much-needed basis for future comparative genomic studies to understand the history of whole genome duplication, gene family evolution, RNA processing and the dynamics of transposable elements. Log in to add this item to your schedule
1 - Boyce Thompson Institute, 533 Tower Road, Ithaca, NY, 14850, USA 2 - National Tsing Hua University, Institute of Molecular & Cellular Biology, Hsinchu, Taiwan 3 - Department of Cell Biology and Molecular Genetics, University of Maryland, Maryland, USA 4 - Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, USA 5 - University Of Maryland, CELL BIOLOGY AND MOLECULAR GENETICS, Biosciences Research Building, 4066 Campus Drive, College Park, MD, 20742, United States 6 - Institute of Molecular & Cellular Biology, National Tsing Hua University, Hsinchu, , Taiwan 7 - Boyce Thompson Institute, 533 Tower Rd, Ithaca, NY, 14853, United States
Keywords: Marsilea vestita chromosome number genome evolution.
Presentation Type: Oral Paper Session: CGT3, Comparative Genomics/Transcriptomics III Location: / Date: Tuesday, July 20th, 2021 Time: 4:00 PM(EDT) Number: CGT3005 Abstract ID:864 Candidate for Awards:Margaret Menzel Award |