Abstract Detail

Comparative Genomics/Transcriptomics

Jin, JianJun [1], Xu, YuXing [2], McKenzie, Patrick [3], Meek, Jared [4], Cohen, Rachel [5], Li, DeZhu [6], Yi, Tingshuang [6], Ree, Richard [7], Eaton, Deren [8].

An annotated chromosome-scale Pedicularis genome assembly enables comparative transcriptomic investigations of floral diversity in the Hengduan Mountains.

The Hengduan Mountains of China is a biodiversity hotspot that harbors the greatest concentration of temperate plant diversity in the world. Among the most diverse plant lineages in this region, few genomic resources are available, which has limited our ability to elucidate the evolutionary processes underlying their rapid radiation. The genus Pedicularis stands out for its exceptional diversity of floral traits displayed across more than 300 endemic species. In this study, we assembled a chromosome-level assembly of P. cranolopha using a combination of long-reads, short-reads, and Hi-C sequencing data. Furthermore, we assembled the transcriptomes of both leaves and flowers of 25 Pedicularis species, representing a broad range of floral and leaf forms. We conducted a comparative transcriptomic analysis, a phylotranscriptomic analysis, and an ancestral gene expression state reconstruction to investigate the underlying gene expression differences among different types of morphologies. Our study will shed new light on the floral morphological evolution of Pedicularis in the Hengduan Mountains, a hotspot of plant diversity.

1 - Columbia University, Ecology, Evolution And Environmental Biology, 1200 Amsterdam Ave, 10th Floor, New York, NY, 10027, United States
2 - Chinese Academy of Sciences, Kunming Institute of Botany, Lanhei Road 132, Kunming, YN, 650204, China
3 - Columbia University, Ecology, Evolution, and Environmental Biology, 10th Floor Schermerhorn Ext., 1200 Amsterdam Ave., New York, NY, 10027, United States
4 - Columbia University, 1200 Amsterdam Ave, New York, NY, 10025, United States
5 - Columbia University, 1190 Amsterdam Ave, New York, NY, 10027, United States
6 - Chinese Academy of Sciences, Lanhei Road 132, Kunming Institute of Botany, Kunming, Yunnan, -, 650204, China
7 - Field Museum Of Natural History, Botany, 1400 S Lake Shore Drive, Chicago, IL, 60605, United States
8 - Columbia University, Ecology, Evolution, And Environmental Biology, 1200 Amsterdam Ave. , Schermerhorn Ext. Office 1007, New York, NY, 10027, United States

Keywords:
Pedicularis
Genome
Transcriptome
floral evolution
Orobanchaceae
Lamiales.

Presentation Type: Oral Paper
Session: CGT2, Comparative Genomics/Transcriptomics II
Location: /
Date: Tuesday, July 20th, 2021
Time: 1:00 PM(EDT)
Number: CGT2003
Abstract ID:749
Candidate for Awards:Margaret Menzel Award