Abstract Detail

Population Genetics/Genomics

Ciotir, Claudia [1], Rubin, Matthew [2], Kantar, Michael [3], Bhakta, Niyati [4], Harris, Zachary [5], Baden, Heide Maria [6], Herron, Sterling A [7], Frawley, Emma [8], Schlautman, Brandon [9], Hübner, Sariel [10], Miller, Allison [11].

Tapping into genomic resources for domestication of Bigleaf lupine (Lupinus polyphyllus Lindl.): a potential perennial food and forage crop of the future.

Growing concerns about global food security, agriculture intensification and climate change have spurred interest in domesticating multifunctional perennial grain crops for the sustainable intensification of agricultural systems. Due to their persistent root systems, perennial grain crops are harvestable over multiple years and offer optimum ecosystem functioning services including soil restoration, water and nutrient retention and utilization. Perennial legumes display pulse and forage agronomic traits that make them good candidates for perennial grain crop domestication. Among wild perennial herbaceous legumes, the native North American Bigleaf lupine species (Lupinus polyphyllus) has been naturalized and domesticated in Europe as a forage (cultivar 'Pervenec') and as a garden ornamental ('Russell Lupins'). Domestication syndrome traits of the Bigleaf lupine were successfully fixed and include dwarf stems, compact inflorescences, indehiscent pods, low alkaloid content, and non-dormant seeds. Screening genetic diversity of candidate species for domestication is a pre-requisite for selecting breeding germplasm. Therefore, the goal of this study was to quantify genetic variation in wild populations available for breeding and assess changes of genetic diversity in populations under domestication. Using genotyping by sequencing (GBS), we generated 13,960 SNPs for 398 individuals of wild populations collected from Colorado, Idaho, and Oregon, ornamental and forage domesticated populations, and feral populations collected from Europe, North America and New Zealand. Genetic analyses suggest that wild populations are highly genetically diverse and well diverged from the domesticated genepool. The structure of most wild populations is shaped by historical evolutionary events including ancestral geneflow, outbreeding, and genetic bottlenecks, thus strong isolation by distance effect was observed among the wild populations. Feral populations are genetically similar to domesticated populations regardless of geographic origin and genetic diversity of most ornamental populations is low, presumably due to continuous inbreeding and selection. In summary, this case study contributes to understanding the genetic basis of domestication in outbreeding perennial grain legumes. The observed diversity among wild and domesticated populations confirms that L. polyphyllus is amenable to two potential strategies for pre-breeding and domestication: de novo pre-breeding from wild germplasm and wide hybridization between wild and promising domesticated cultivars. The studied germplasm collection can serve as a resource for breeding advanced and high-yielding L. polyphyllus new varieties.

1 - Missouri Botanical Garden, 5800 Highlands Plaza Drive, #112, Saint Louis, MO, 63110, United States
2 - Donald Danforth Plant Science Center, 975 North Warson Road, Miller Lab, St. Louis, MO, 63132, United States
3 - University Of Hawaii, Tropical Plant And Soil Sceinces, St. John Plant Science Lab, Room 102, 3190 Maile Way , Honolulu, HI, 96822, United States
4 - The Donald Danforth Plant Science Center, 975 N Warson Rd, Olivette, Saint Louis, MO, 63132, USA
5 - Saint Louis University, Biology, 1 N. Grand Blvd, Saint Louis, MO, 63103, United States
6 - University of Southern Denmark, Biology, Campusvej 55 , Odense M, 5230, Denmark
7 - Donald Danforth Plant Science Center, Allison Miller Lab, 975 N Warson Road, Saint Louis, MO, 63132, United States
8 - Washington University In St. Louis, Biology, 1 Brookings Drive, McDonnell Hall, St. Louis, MO, 63130, United States
9 - The Land Institute, 2440 E Water Well Rd, Salina, KS, 67401, USA
10 - MIGAL - Galilee Research Institute, 1 Tarshish St., Kiryat Shmona, 11016, Israel
11 - Saint Louis Univ./Danforth Plant Science Center, Biology, 3507 Laclede Avenue, Macelwane Hall, St. Louis, MO, 63110, United States

Keywords:
de novo domestication
evolution
germplasm
genetic diversity
lupine
Population genomics
perennial grain crops
sustainable intensification.

Presentation Type: Oral Paper
Session: PGG3, Population Genetics and Genomics III
Location: /
Date: Wednesday, July 21st, 2021
Time: 1:45 PM(EDT)
Number: PGG3006
Abstract ID:744
Candidate for Awards:Margaret Menzel Award