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Cantillo, Johanna [1], Duncan, Keith [1], Kwasniewski, Misha [2], Londo, Jason [3], Maimaitiyiming, Matt [4], Rubin, Matthew [5], Miller, Allison [6].

Architectural differences in rootstocks are associated with changes in shoot system traits in grafted grapevines.

Grafting is an ancient horticultural technique that fuses the root system of one plant to the shoot system of another plant. To characterize rootstock root architecture and influences on shoot system phenotypes in grapevines, we conducted a greenhouse experiment in which the grapevine scion ‘Marquette’ was grafted to five rootstocks ‘Freedom’, ‘5C’, ‘1103P’, ‘3309C’, and ‘SO4’ in replicates of 12. Grafted plants were planted in pots at Day 1, split into two groups (control, drought) at Day 33, and transitioned to recovery at Day 49. To characterize root architecture prior to and following stress, 3D root architecture was quantified at the beginning and end of the experiment. Differences in architecture were subtle initially, but following drought treatment both average root radius and specific root length reflected differences based on rootstock genotype. To assess root-system impacts on shoot-system phenotypes, plant size, physiology, leaf elemental composition, and leaf volatiles were measured. ‘Marquette’ grafted to ‘1103P’ and ‘5C’ exhibited reduced stomatal conductance, transpiration, photosynthetic rate relative to ‘Marquette’ grafted to ‘3309C’, ‘Freedom’, and ‘SO4’. Just under 50% of variance in the concentrations of Calcium, Cadmium, Copper, and Potassium in ‘Marquette’ leaves were attributed to rootstock genotype. In addition, volatiles emitted by leaves reflected rootstock genotype and treatment interaction. This study demonstrates that grapevine rootstock cultivars differ in root architecture, and that differences become more pronounced under water stress. Differences in rootstocks are associated with concomitant changes in scion size, physiology, elemental composition, and leaf volatiles; further, for most traits rootstock impact on shoot system phenotypes is amplified under stress. These data highlight natural variation in root system architecture and its implications for shoot system phenotypes in grafted grapevines.

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Related Links:
Miller Lab Website

1 - Danforth Plant Science Center, Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, Missouri, 63132, United States
2 - Pennsylvania State University
3 - USDA ARS, 15 Castle Creek Drive, 15 Castle Creek Drive, Geneva, NY, 14456, United States
4 - University of Missouri
5 - Donald Danforth Plant Science Center, 975 North Warson Road, Miller Lab, St. Louis, MO, 63132, United States
6 - Saint Louis Univ./Danforth Plant Science Center, Biology, 3507 Laclede Avenue, Macelwane Hall, St. Louis, MO, 63110, United States

root shoot interaction
root architecture.

Presentation Type: Oral Paper
Session: PHYS, Physiology
Location: /
Date: Wednesday, July 21st, 2021
Time: 11:30 AM(EDT)
Number: PHYS007
Abstract ID:668
Candidate for Awards:None

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