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Abstract Detail

Development and Structure

Da Silveira, Sylvia Rodrigues [1], Le Gloanec, Constance [1], Routier-Kierzkowska, Anne-Lise [1], Kierzkowski, Daniel [1].

Live-imaging uncovers cellular growth patterns shaping Arabidopsis stamen.

One of the fundamental questions in biology is how organ shapes are acquired during the development of multicellular organisms. Precise understanding of organogenesis requires measurements of cellular behaviors over space and time. In plants, such a quantitative approach has been successfully used to dissect organ development both in leaves and external floral organs such as sepals. However, the observation of floral reproductive organs is hampered as they develop inside tightly enclosed floral buds, difficult to access for imaging. Here we develop a new confocal time-lapse imaging method that allows, for the first time, a full quantitative characterization of the development of stamens, male reproductive organs, from their initiation until the final shape is established post-anthesis. Lineage tracing reveals the early specification of the filament and the anther. Formation of the anther lobes is associated with a temporal increase of growth at the lobes with simultaneous repression of growth in the notches. Filament elongation passes through three phases: (1) Intense anisotropic growth and high proliferation right after specification; (2) Restriction of growth and proliferation to the filament base, which elongates relatively slowly and thickens; (3) Resumption of intense and anisotropic growth, displaced to the distal portion of the filament, without cell proliferation. The full quantitative analysis of growth achieved here provides a solid framework for future studies into stamen development and its adaptation to environmental perturbations.

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1 - IRBV, University of Montreal, Department of Biological Sciences, 4101 Sherbrooke St E, Montreal, QC, H1X 2B2, Canada

Time-lapse imaging
Arabidopsis thaliana
Growth analysis  
Floral organ.

Presentation Type: Oral Paper
Session: DS1, Development and Structure I
Location: /
Date: Tuesday, July 20th, 2021
Time: 1:15 PM(EDT)
Number: DS1013
Abstract ID:596
Candidate for Awards:None

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