Abstract Detail

Phylogenomics

Sassone, Agostina [1], Blattner, Frank [2].

Plastome phylogenetics within the South American garlics (tribe Leucocoryneae, Allioideae, Amaryllidaceae).

The tribe Leucocoryneae has widely distributed in South America including ca. 200 species. The circumscription of the genera and species within the tribe has been problematic, being  Nothoscordum,  the most abundant and taxonomical challenge genus. Even though there have been recent publications focused on complete chloroplast genome sequences analysis of Allioideae, sampling was focused on the genus  Allium  and lack proper representation of the other tribes of the subfamily. The aims of our study are to 1) characterize the organization of the complete cp genomes of the genera included in the Leucocoryneae 2) generate a comprehensive and deep phylogeny including all lineages of the subfamily focused on the genus  Nothoscordum. We used a whole-genome shotgun (WGS) to recover the complete plastome of the samples. We newly sequenced 70 specimens and combined them with sequences available in GenBank.  De novo  plastomes  assemble was conducted with GetOrganelle, and when not possible, they were assembled using a reference sequence with NOVOPlasty. Phylogenetic analysis was inferred with RAxML. The plastome of the newly analyzed species presents a quadripartite structure that includes two inverted repeat regions, a small single-copy region (SSC), and a large single-copy region (LSC) varying in size from 153,303 (Allium triquetrum) to 156,770 bp (Beauverdia vitatta). Our molecular phylogenomic results strongly support the monophyly of the tribe Leucocoryneae. The genus  Leucocoryne  and  Latace  resulted as sister genera. Within  Tristagma  two lineages are recovered (one of them including  Ipheion). Nothoscordum  resulted in the most recently diversified genus, and two major clades are recovered (one including species of  Beauverdia). Our talk will present these results and discuss biogeographic patterns within Allioideae. We will compare the topologies obtained from this plastome dataset with those previously obtained by Genotyping by Sequencing to assess hybridization, as well as other evolutionary processes involved in shaping the current diversity of the South American garlics.

1 - IPK Gatersleben, Corrensstr. 3, Corrensstr. 3, Stadt Seeland OT Gatersleben, ST, 06466, Germany
2 - IPK Gatersleben, Corrensstr. 3, Gatersleben, Seeland , Sachsen Anhalt, 06466, Germany

Keywords:
Alliaceae
chloroplast
phylogenomics
whole genome shotgun (WGS).

Presentation Type: Oral Paper
Session: PHYLOIII, Phylogenomics III
Location: Virtual/Virtual
Date: Thursday, July 22nd, 2021
Time: 10:45 AM(EDT)
Number: PHYLOIII004
Abstract ID:569
Candidate for Awards:None