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Maglie, Michele [1], Baldisserotto, Costanza [1], Ibáñez González, María José [2], Mazzuca Sobczuk, Tania [2], Sabia, Alessandra [1], Pancaldi, Simonetta [1].

Effects of co-cultivation and red LEDs light on growth and lipid content of Tisochrysis lutea and Nannochloropsis oculata.

Microalgae have taken considerable importance because of their wide application range and their ability to produce high added value biomolecules (lipids, proteins, pigments, etc.) for nutraceutical, cosmeceutical and pharmaceutical applications (Rizwan et al. 2018). New technologies for microalgae cultivation aim to reduce costs and environmental impact, improve the yield, and reduce water use and nutrient supply (Levasseur et al. 2020). With these purposes, some studies have been focused on co-cultivations of different microalgae; this technique could also allow to produce, in a single cultivation process, a mixture of biomolecules characteristic of the species used (Rashid. et al. 2019). In this study, the co-cultivation of Tisochrysis lutea and Nannochloropsis oculata, two strains of microalgae interesting for their composition in pigments and fatty acids, was tested in two lighting conditions. Mono-cultures of the two species were set up as controls. Both mono- and co-cultures were cultivated for 18 days in SWES medium and exposed to a combination of 60 μmolphotons m−2s−1 of red (peak at 660 nm) plus 40 μmolphotons m−2s−1 of white LED lights (colour temperature 4000 K). Cultures exposed to 100 μmolphotons m−2s−1 of white LEDs have been used as controls. Under both light conditions, co-cultivation negatively affected the growth of T. lutea. On the contrary, co-cultivation stimulated the growth of N. oculata and, at the end of experiment, white light co-cultivated cells had a significant higher concentration than mono-cultures. This also occurred in red-enriched light cultures, although the difference was not statistically significant. Spectrophotometric analyses of the photosynthetic pigments revealed no significant differences attributable to red-enriched light treatments. The fatty acids extractions and analyses by gas chromatography were performed on samples of mono- and co-cultures in both light conditions. Red-enriched light treatments improved the total fatty acids content (% dw) in mono-cultures of N. oculata, increasing from 9.0% (±0.4) in white light to 11.5% (±0.1) in red-enriched light cultures. However, the best results were obtained in co-cultures: from 8.3% (±0.3) to 18.9% (±0.2). In mono-cultures of T. lutea the increase of the total fatty acid content was not significant, nevertheless the relative percentage of the ω-3 docosahexaenoic acid (DHA) increased by 72% in cultures exposed to red-enriched light compared to those exposed to white light.

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1 - University of Ferrara, Department of Life Sciences and Biotechnology, Via L. Borsari, 46, Ferrara, FE, 44121, Italia
2 - University of Almería, Department of Chemical Engineering, Almería, Spain

Fatty acids
Tisochrysis lutea
Nannochloropsis oculata.

Presentation Type: Poster
Session: P3, Phycology Posters
Location: Virtual/Virtual
Date: Wednesday, July 21st, 2021
Time: 5:00 PM(EDT)
Number: P3PY001
Abstract ID:227
Candidate for Awards:None

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