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Abstract Detail

Hybrids and Hybridization

Hussain, Sidra [1], Winter, David [1], Cox, Murray [1], Soltis, Douglas E. [2], Soltis, Pamela S. [3], Tate, Jennifer [1].

Evolution of Cytonuclear Coordination in Tragopogon (Asteraceae) Allopolyploids.

Allopolyploidy is considered a major pathway for diversification of species and has generated many of the world’s most important crops. In allopolyploids, the duplicated and biparentally inherited nuclear genomes interact with only one set of maternally inherited cytoplasmic (mitochondrial and plastid) genomes, which results in an altered cytonuclear stoichiometry. However, cytonuclear genome interactions in allopolyploids remain underexplored. Synchronization between nuclear and cytoplasmic genomes is essential for regulation of important developmental processes, such as respiration and photosynthesis in plants, and thus is an important aspect to allopolyploid genome stability. The aim of this study is to determine how these potential genome imbalances hinder or aid speciation in allopolyploids. In this project, we are employing RNA CaptureSeq to examine expression differences in Tragopogon miscellus allopolyploids. These are young, naturally occurring allopolyploids that have formed multiple times, including reciprocally. Using the parental T. dubius draft genome, we developed probes for ~310 loci involved in nuclear-mitochondrial and nuclear-plastid complexes. We also used annotated genomes of Arabidopsis thaliana and Lactuca sativa to determine protein coding sequences. The gene expression data from RNA CaptureSeq experiment will be presented. As these allopolyploids formed recently and recurrently, they offer a window into the potential for repeated evolution of cytonuclear coordination. Using this model system, we will be able to determine how organellar and nuclear genomes coordinate to facilitate speciation in plants.

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1 - Massey University, School Of Fundamental Sciences, Private Bag 11222, Palmerston North, MWT, 4442, New Zealand
2 - University of Florida, Department of Biology, Gainesville, FL, 32611, United States
3 - University Of Florida, Florida Museum Of Natural History, PO Box 117800, Gainesville, FL, 32611, United States

RNA CaptureSeq
Cytonuclear interactions
Tragopogon miscellus.

Presentation Type: Oral Paper
Session: HH1, Hybrids and Hybridization I
Location: /
Date: Wednesday, July 21st, 2021
Time: 10:45 AM(EDT)
Number: HH1004
Abstract ID:215
Candidate for Awards:Margaret Menzel Award

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