| Abstract Detail
Recent Topics Posters Kaur, Harleen [1], Ozga, Jocelyn A. [1], Wadhuthanthri, Kosala D. [1], Reinecke, Dennis M. [1]. Gibberellin regulates starch metabolism in the developing seeds of Pisum sativum L. . Gibberellins (GAs), a key plant hormone class, control seed growth and development in pea. The overexpression of PsGA3ox1 in the semi-dwarf cultivar ‘Carneval’ (TG1 line) increased the level of bioactive GA1 and produced 10 to 23% larger seed size (particularly embryo) during the seed-filing development period (8 to 20 days after anthesis, DAA) and at maturity than did the control C1 plants. Greater embryo growth and enhanced starch levels were observed in TG1 seeds from 14-20 DAA compared to that of C1. ADP-glucosepyrophosphorylase (AGPase) is a key rate-limiting enzyme that regulates starch biosynthesis in plants. To understand the role of GAs in seed development, the enzyme activity and expression profile of AGPase was studied in C1 and TG1 seed coat and cotyledon tissues during the seed-filling development period. At 12 DAA, starch begins to accumulate in the rapidly developing cotyledons and increases with development. In the cotyledons, the increased expression levels of PsAGPS1 (the most abundant small subunit of AGPase) from 12-16 DAA and PsAGPS2 at 12 DAA were associated with increased AGPase enzyme activity during this period in TG1 compared to that C1. These data suggest that GAs can enhance starch accumulation in the cotyledons by upregulating expression of AGPase.
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1 - University of Alberta, Department of Agricultural, Food, and Nutritional Science, Plant BioSystems Group, Edmonton, AB, T6G 2P5 , Canada
Keywords:
gibberellins
seed development
starch
ADP-glucosepyrophosphorylase.
Presentation Type: Recent Topics Poster
Session: P1, Recent Topics Posters
Location: Virtual/Virtual
Date: Thursday, July 22nd, 2021
Time: 5:00 PM(EDT)
Number: P1RT035
Abstract ID:1426
Candidate for Awards:None |
