Abstract Detail

Comparative Genomics/Transcriptomics

Pradhan, Bijaya [1], Nelson, Dillon [1], Neupane, Surendra [2], Nepal, Madhav [3].

Characterization of GmWRKYs involved in a 3-way Interaction of Soybean, Soybean-Aphid and Soybean-Cyst-Nematode.

Soybean aphid (SBA) and Soybean-cyst-nematode (SCN) are the two major pests of soybean,   affecting soybean production. One recent study has reported one WRKY gene (GmWRKY) promoting SCN resistance in soybean, however, GmWRKYs involved in   a three-way interaction of soybean, SBA and SCN are yet to be charcaterized. In the present study, we aimed to determine the GmWRKYs involved in such interaction. A greenhouse water bath experiment was conducted, and pathogen interactions were examined in randomized block design. The SCN-resistant and SCN susceptible soybean cultivars were used with SBA, SCN, and both SCN and SBA. The treatments receiving SCN were infested with 2000 nematode eggs, and 15 (biotype1) SBA were infested with the treatments receiving soybean-aphids at the second trifoliate growth stage. Differentially expressed genes using the biotic stress pathway into MapMan showed that the plants treated with SBA and SCN in resistant cultivars showed the involvement of soybean WRKYs (GmWRKYs) in gene regulation. Twelve GmWRKYs were up-regulated at five days post infestation (dpi), and 14 GmWRKYs were down-regulated in 30 dpi. Altogether, we found 26 GmWRKYs involved in gene regulation in the 3-way interaction. Validation of these genes using qRT- PCR data and statistical analysis are under progress. In this paper, we will discuss the final outcomes of the qPCR data and determination of the GmWRKYs of the major effects in the three-way interaction.

Related Links:
Nepal Lab at South Dakota State University

1 - South Dakota State University, McFadden Biostress Laboratory, Room 213, Brookings, SD, 57007, United States
2 - University of Florida, UF/IFAS Citrus Research and Education Center, Institute of Food and Agricultural Sciences, Gainesville , FL, 33850, USA
3 - South Dakota State University, Biology And Microbiology, McFadden Biostress Laboratory, South Dakota State University, Brookings, SD, 57007, United States

Keywords:
WRKY genes  
Soybean-SA-SCN intercation  
abiotic and biotic stress
Plant stress regulation  .

Presentation Type: Poster
Session: P1, Comparative Genomics/Transcriptomics Posters
Location: Virtual/Virtual
Date: Monday, July 19th, 2021
Time: 5:00 PM(EDT)
Number: P1GT014
Abstract ID:998
Candidate for Awards:None