Abstract Detail

Ecophysiology

Ray, Dustin [1], Savage, Jessica [1].

Quantification of seasonal changes in sieve tube conducting area of Populus balsamifera stems with the LM26 antibody.

Carbon transport in the phloem is necessary to support plant growth and metabolism, but little is known about the seasonal changes within the phloem structure to support carbon allocation. Understanding the seasonal dynamics of carbon allocation and specifically sap flow require the identification of actively conducting sieve elements, which is difficult, time consuming, and expensive given the current techniques to confidently identify active sieve elements (transmission electron microscopy, magnetic resonance imaging, callose staining, etc.). The recent identification of a monoclonal antibody (LM26) that binds an epitope present on a pectin that appears to be specific to sieve element walls has opened a new avenue for identification and quantification of sieve elements. Since immunodetection by the LM26 epitope appears to be seasonal in Populus, it presents a unique tool with which to quantify the seasonally changing conductive area of the sieve elements within the phloem. We quantified the sieve conducting area within 3-year-old stems while recording the timing of major floral and foliar phenological events, to determine how the phloem conducting capacity changes seasonally and present preliminary data relating changes in phloem conducting capacity to plant phenology.

1 - University of Minnesota Duluth, Department of Biological Sciences, 1035 Kirby Drive 207 Swenson Science Building, Duluth, MN, 55812, USA

Keywords:
Phloem Transport
phloem  
sieve element
Populus
seasonal change
seasonality.

Presentation Type: Poster
Session: P2, Ecophysiology Posters
Location: Virtual/Virtual
Date: Tuesday, July 20th, 2021
Time: 5:00 PM(EDT)
Number: P2PE002
Abstract ID:752
Candidate for Awards:None