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Allen, Geraldine [1], Liston, Aaron [2], Eriksson, Torsten [3].

Phylogenetic relationships of the strawberry subtribe Fragariinae (Rosaceae, Potentilleae) inferred from whole genome sequences.

New approaches to analyzing large DNA data sets can resolve long-standing questions in phylogenetically complex groups. In the Rosaceae, the tribe Potentilleae has long been considered problematic, presenting difficulties in delimiting genera and identifying monophyletic groups. It consists of two clades: i) Potentilla and its segregate genera, and ii) the approximately 14 genera of the subtribe Fragariinae. Here we focus on relationships within the Fragariinae. We obtained whole genome sequences from 25 samples in 12 genera. We recovered genes using HybPiper, with target sequences consisting of 78 chloroplast and 12353 nuclear genes of Fragaria vesca. We obtained on average all 78 chloroplast genes and 8500 nuclear genes (range 2300-11750) from the sampled species. After filtering out low-quality gene alignments, we performed maximum likelihood analyses using IQ-TREE. These revealed at least two well-supported subclades in both plastid and nuclear trees, i) the Drymocallis subclade (Chamaecallis, Chamaerhodos, Dasiphora, Drymocallis and Potaninia) and ii) the Sibbaldia subclade (Sibbaldia, Sibbaldiopsis and Sibbaldianthe); a third subclade including Alchemilla (represented here by Aphanes) and Farinopsis was sister to the Sibbaldia group. Differences between the chloroplast and nuclear gene trees, as well as low gene and site concordance factors for several branches, indicated conflict. This could reflect incomplete lineage sorting, but reticulation and polyploidy are also likely. Polyploids are common in the Fragariinae, especially in the Alchemilla and Sibbaldia subclades. Although several species of Sibbaldia have previously been found to belong to Potentilla, relationships among the remaining taxa of this subclade remain poorly understood. Sibbaldia species are diploid and Sibbaldiopsis is polyploid; in our analyses, Sibbaldiopsis was either nested within Sibbaldia (plastid tree) or the reverse (nuclear tree). Two other genera not included in the main subclades, Comarum and Fragaria, both differed in their positions in plastid and nuclear trees. The strawberry genus Fragaria was sister to the Drymocallis subclade in the plastid phylogeny and to the Sibbaldia + Alchemilla subclades in the nuclear phylogeny, and has been suggested elsewhere as the sister group to the rest of the Fragariinae.

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1 - University Of Victoria, Department Of Biology, PO Box 1700 Station CSC, Victoria, BC, V8W 2Y2, Canada
2 - Oregon State University, Department Of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, OR, 97331, United States
3 - University Of Bergen, University Museum, Department Of Natural History, Postboks 7800, Bergen, N-5007 , Norway

whole genome sequencing.

Presentation Type: Oral Paper
Session: PHYLOIV, Phylogenomics IV
Location: Virtual/Virtual
Date: Friday, July 23rd, 2021
Time: 12:30 PM(EDT)
Number: PHYLOIV001
Abstract ID:702
Candidate for Awards:None

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