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Van Wyk, Judson [1], Wang, Xinxin [1], Childs, Kevin [2], Bonito, Gregory [1].

Omics analysis of the cultivated Morel, Morchella rufobrunnea.

Morels, genus Morchella, are among the most popular edible mushrooms in the world but few species have been cultivated. Currently, wild foraged morels are heavily relied upon to meet the demands of the market. Though some species have been cultivated, inconsistencies in yield have kept them economically infeasible to produce commercially. M. rufobrunnea, commonly found in landscaping wood chips, was the first morel cultivated indoors and is part of the most basal Morchellaceae clade. Due to its commercial value, M. rufobrunnea is a clear target for an omics-based analysis of its development. In order to investigate the genes associated with each stage of ascocarp development, transcriptomes of the mycelium, sclerotia, conidia, primordia and mature ascocarps were sequenced using Oxford Nanopore Technology (ONT) RNA-seq. A denovo genome was assembled using ONT and Illumina-hiseq, and has an estimated genome size of 60 Mb. This work will be used to assess important genes in Morchella development which can be used to inform future cultivation efforts in the genus.

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1 - Michigan State University, Plant, Soil, and Microbial Sciences, Molecular Plant Sciences Building, 1066 Bogue Street Rm 3270, East Lansing, MI, 48824, USA
2 - Michigan State University, Plant Biology, 1130 Molecular Plant Sciences Building, East Lansing, MI, 48824, USA


Presentation Type: Poster
Session: MYP2, Mycology Posters II
Location: Virtual/Virtual
Date: Tuesday, July 20th, 2021
Time: 5:00 PM(EDT)
Number: MYP2002
Abstract ID:1070
Candidate for Awards:MSA Best Poster Presentation Award by a Graduate Student


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