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Abstract Detail

Comparative Genomics/Transcriptomics

Adams, Keith [1].

Progenitor-inclusive transcriptome profiling of polyploid Brassica napus reveals subgenome-dominant gene expression and alternative splicing in response to to Sclerotinia sclerotiorum.

Polyploidy events have played an extensive role in the evolution of flowering plants. Allopolyploids can show rapid and novel transcriptome changes including novel alternative splicing patterns. The extent to which abiotic stress modulates alternative splicing remains a nascent topic in polyploidy research. We subjected both natural and resynthesized lines of polyploid Brassica napus, along with their parents Brassica rapa and Brassica oleracea, to infection with the fungal pathogen Sclerotinia sclerotiorum. RNA-seq analyses revealed widespread divergence between polyploid subgenomes in terms of both constitutive gene expression and alternative splicing patterns. This manifested in a global expression bias towards the B. oleracea-derived (C) subgenome in both resynthesized and natural B. napus, enhanced by widespread non-parental down-regulation of the B. rapa-derived (A) homeolog. In the resynthesized B. napus specifically, this resulted a disproportionate C subgenome contribution to plant innate immunity and pathogen defense response, characterized by biases in both transcript expression level and the proportion of induced genes.

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1 - Boise State University, Snake River Plains Herbaria, , Boise, Idaho, 83725, USA

gene expression
duplicated genes
Sclerotinia sclerotiorum
alternative splicing
abiotic and biotic stress

Presentation Type: Oral Paper
Session: CGT2, Comparative Genomics/Transcriptomics II
Location: /
Date: Tuesday, July 20th, 2021
Time: 1:30 PM(EDT)
Number: CGT2005
Abstract ID:1065
Candidate for Awards:None

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