Abstract Detail

Phylogenomics

Odufuwa, Phebian [1], Smith, James [1], Mansfield, Donald [2], Feist, Mary Ann [3], Darrach, Mark [4], Downie, Stephen [5], Plunkett, Gregory [6].

Diversification in the Perennial Endemic North American Clade of Apiaceae – How far are we from completion?

The impact and role that native herbaceous plants play in the natural ecosystem cannot be overemphasized. They not only help in maintaining the populations of insect-herbivores that depend on them for food, but they also help in preserving pollinators that are useful for agricultural landscapes. The Perennial Endemic North American Clade of Apiaceae (PENA) which contains ~252 species in 19 artificial genera, is one such group. Despite its ecological and cultural importance, a fully resolved, well-supported phylogenetic tree of the entire clade is still lacking.   This hinders the prioritization of many of its species for conservation efforts because we are still uncertain where species, or even generic boundaries in this group are. My presentation will provide an update on the progress made so far towards providing a phylogenetic framework and delineating species boundaries of all the taxa that constitute PENA. The group was previously thought of as being a monophyletic group, however, previous phylogenetic studies using Sanger sequence data have shown polytomies and polyphyly in many of the previously recognized genera within PENA. Recent phylogenetic studies using Next-generation sequence data have also shown some evidence that PENA is not a monophyletic clade.   These problems may be the result of several factors including incipient speciation, convergent evolution, hybridization and introgression, and incomplete lineage sorting. Therefore, we opted to use the next-generation target capture technique that employs the Angiosperms 353 probe set to collect low-copy nuclear gene sequences from the genomes of each PENA species to resolve evolutionary relationships among these taxa. The results from concatenated datasets of 67 PENA species and one outgroup were somewhat in congruence with previous studies that used Sanger data. We will continue our effort in getting all the species of PENA into the phylogenetic framework. Discrepancies may be due to hybridization or incomplete lineage sorting and we will investigate coalescent methods in the future.

1 - BOISE STATE UNIVERSITY, Department Of Biological Sciences, 1910 University Drive, Ms1515, Boise, ID, 83725, United States
2 - College of Idaho, Biology, 2112 Cleveland Boulevard, Caldwell, ID, 83605, USA
3 - University Of Wisconsin/ Wisconsin State Herbarium, Botany, 430 Lincoln Dr., Birge Hall, Madison, WI, 53706, United States
4 - University of Washington, Corydalis Consulting, Burke Museum, Indianola, WA, USA
5 - University of Illinois, Plant Biology, Urbana, IL, USA
6 - New York Botanical Garden, Cullman Program For Molecular Systematics, 2900 Southern Blvd., Bronx, NY, 10458, United States

Keywords:
Apiaceae
PENA
convergent evolution
NGS
phylogenetic framework
species delimitation
generic circumscription.

Presentation Type: Oral Paper
Session: PHYLOII, Phylogenomics II
Location: Virtual/Virtual
Date: Tuesday, July 20th, 2021
Time: 4:00 PM(EDT)
Number: PHYLOII005
Abstract ID:411
Candidate for Awards:None