| Abstract Detail
Development and Structure Dunivant, Taryn [1], Singh, Vibhuti [1], Livingston, Kaylee [1], Hileman, Lena [1]. CYC’d Out: CYCLOIDEA paralogs exhibit redundant function in establishing dorsal petal identity in Mimulus lewisii. Repeated independent transitions between radial and bilateral flower symmetry have occurred across the angiosperm phylogeny, contributing to the vast diversity we see in floral morphology. The genetic program for bilateral flower symmetry has been documented in the model system Antirrhinum majus where the paralogs CYCLOIDEA (CYC) and DICHOTOMA (DICH) have partially redundant functions in establishing dorsal petal identity. These paralogs resulted from a duplication event in the ECE-CYC2 gene lineage. Within Lamiales, at least 12 additional duplications in the ECE-CYC2 lineage have occurred. The close homologs CYC1 and CYC2 resulted from one of these additional duplication events at the base of the higher core Lamiales. In this study, we are using the emerging model Mimulus lewisii, to test for conservation of MlCYC1 and MlCYC2 in flower symmetry development, and whether these genes similarly function redundantly. Using Agrobacterium-mediated stable transformation techniques and RNA interference (RNAi), we were able to characterize MlCYC1 and MlCYC2 RNAi silenced lines. In addition, by cross pollinating MlCYC1 and MlCYC2 single lines, we generated and characterized double MlCYC1:MlCYC2 RNAi silenced lines. Our results from RNAi silencing, and consistent with our gene expression analyses, demonstrate that MlCYC1 and MlCYC2 together function to specify dorsal flower identity. Additionally, we find extensive functional redundancy between the paralogs MlCYC1 and MlCYC2, with MlCYC1 playing the dominant role in establishing dorsal petal identity. These results are consistent with what is found in A. majus, nonetheless the paralogs arose from independent gene duplication events in the ECE-CYC2 gene lineage.
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1 - University of Kansas, Ecology and Evolutionary Biology, 1200 Sunnyside Ave, Lawrence, KS, 66045, United States
Keywords:
Mimulus lewisii
CYCLOIDEA
Bilateral flower symmetry
Stable transformation
RNAi Silencing.
Presentation Type: Oral Paper
Session: DS2, Development and Structure II
Location: /
Date: Wednesday, July 21st, 2021
Time: 11:00 AM(EDT)
Number: DS2005
Abstract ID:287
Candidate for Awards:Katherine Esau Award,Maynard F. Moseley Award,Developmental and Structural Section Graduate Student Registration Award |
