| Abstract Detail
Recent Topics Posters Giebelhaus, J. Duncan [1], Ozga, Jocelyn A. [2], Reinecke, Dennis M. [2]. Gibberellin regulation of protein accumulation in developing pea (Pisum sativum L.) seeds. Many field pea varieties have a mutation in the PsGA3ox1 gene which causes a decrease in bioactive gibberellins (GAs), a plant hormone that regulates growth and development. This mutation in field pea leads to lower GA levels, producing shorter stemmed plants useful for cultivation; however, its effects on seed composition are not well understood. This study tests the hypothesis that part of GAs effect on seed development is through modulation of protein accumulation in the developing seeds. Using GA overproducing and isogenic null control lines, changes in seed tissue free amino acid and total nitrogen content were determined to identify potential GA-induced effects on processes that affect protein accumulation during seed development. Cotyledon nitrogen content per seed and estimated protein content (per mg dry weight) were elevated in the GA-overproducing line during development and at maturity compared to the null line, suggesting that seed storage protein accumulation is influenced by GA. Developmental variation in the profiles of key free amino acids involved in seed nitrogen transport and storage in seed coat, endosperm, and cotyledon seed tissues indicate that GA could potentially regulate amino acid transport and metabolism dynamics within developing seeds. These modifications, in turn, could influence the rate of storage protein synthesis in the cotyledons with possible implications on final seed protein content. The knowledge gained on GA regulation of storage protein production during seed development can be used to improve protein content in conventional field pea varieties, which could address issues faced by global agriculture and the plant-protein industry.
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1 - 502 10405 Saskatchewan Drive, Edmonton, AB, T6E 4R9, Canada
2 - University of Alberta, Plant BioSystems Division, Department of Agricultural, Food, and Nutritional Science, Edmonton, AB, T6G2P5, Canada
Keywords:
Gibberellin
plant reproduction
Protein
pea.
Presentation Type: Recent Topics Poster
Session: P1, Recent Topics Posters
Location: Virtual/Virtual
Date: Thursday, July 22nd, 2021
Time: 5:00 PM(EDT)
Number: P1RT028
Abstract ID:1418
Candidate for Awards:None |
