Abstract Detail

Recent Topics Posters

Oulo, Millicent [1], Yang, Jia-Xin Yang [1], Dong, Xiang [1], Wanga, Vincent Okelo [2], Mkala, Elijah Mbandi [2], Munyao, Jacinta Ndunge [1], Onjolo, Victor Omondi [1], Rono, Peninah Cheptoo [1], Hu, Guang-Wan [1], Wang, Qing-Feng [1].

Complete Chloroplast Genome of Rhipsalis baccifera displays Genome Rearrangement, Intron Gain and Loss: insights to its Phylogenetic history.    .

Family Cactaceae is almost endemic to the New World except for Rhipsalis baccifera that naturally occurs in both the New World and the Old World. Though this species has thus drawn the attention of most researchers, the molecular work is still insufficient. The complete chloroplast (cp) genome of R. baccifera is reported here for the first time. The cp genome of R. baccifera has 122, 333 base pairs (bp), with a large single-copy (LSC) region (81,459 bp), SSC (23,531 bp), and two inverted repeats (IR) regions each 8530 bp. The genome contains 110 genes, with 73 protein-coding genes, 31 tRNAs, 4 rRNAs, and 2 pseudogenes. Twelve genes have introns, with loss of introns being observed in, rpoc1 clpP and rps12 genes. 49 repeat sequences and 62 simple sequence repeats (SSRs) were found in the genome. Comparative analysis with eight species of the ACPT (Anacampserotaceae, Cactaceae, Portulacaceae, and Talinaceae) clade of the suborder Portulacineae species, showed that R. baccifera genome has a higher number of rearrangements, with a 19 gene inversion in its LSC region representing the most significant structural change in terms of its size. Inversion of the SSC region seems common in subfamily Cactoideae, and another 6 kb gene inversion between rbcL- trnM was observed in R. baccifera and Carnegiea gigantea. The IRs of R. baccifera are contracted. The phylogenetic analysis among 36 complete chloroplast genomes of Caryophyllales species and two outgroup species supported monophyly of the families of the ACPT clade. R. baccifera occupied a basal position of the family Cactaceae clade in the tree. A high number of rearrangements in this cp genome suggests a larger number of mutation events in the history of evolution of R. baccifera. These results provide important tools for future work on R. baccifera and in the evolutionary studies of the suborder Portulacineae.

1 - Wuhan Botanical Garden, University of Chinese Academy of Sciences, Botany, 1#Lumo Road, Moshan Wuchang, Wuhan, Hubei Province, P.R.China, Wuhan, Hubei, China
2 - Wuhan Botanical Garden, University of Chinese Academy of Sciences, Botany, 1#Lumo Road, Moshan Wuchang, Wuhan, Hubei Province, P.R.China, Wuhan, China

Keywords:
Rhipsalis baccifera
Cactaceae
rearrangements
phylogeny.

Presentation Type: Recent Topics Poster
Session: P1, Recent Topics Posters
Location: Virtual/Virtual
Date: Thursday, July 22nd, 2021
Time: 5:00 PM(EDT)
Number: P1RT013
Abstract ID:1403
Candidate for Awards:None